Regulatory

Part:BBa_I720005:Design

Designed by: Andrew Cheng   Group: iGEM07_Michigan   (2007-10-26)

NifA lacI RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3235
    Illegal PstI site found at 118
    Illegal PstI site found at 217
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3236
    Illegal PstI site found at 118
    Illegal PstI site found at 217
    Illegal PstI site found at 3250
    Illegal NotI site found at 3243
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3236
    Illegal PstI site found at 118
    Illegal PstI site found at 217
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3236
    Illegal PstI site found at 118
    Illegal PstI site found at 217
    Illegal PstI site found at 3250
    Illegal AgeI site found at 3108
    Illegal AgeI site found at 3220
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 463


Design Notes

NifA has two PstI sites (biobrick site). We had to insert NifA (cut with EcoRI and SpeI) in front of lacI and RFP to avoid cutting with PstI. Any future projects involving this plasmid needs to take this into account.


Source

NifA comes from Klebsiella Pneumonia genomic DNA. lacI and RFP come from biobricks.

References